Any condition that has an element of inflammation has a good chance of being rooted in the gut.  And Zonulin is the parameter we are looking at to give us that information.  And when it is elevated, we have the condition necessary to create inflammation leading to reduced health and disease.

One thing that can be done to lower the level of Zonulin is to use immunoglobulins which can come from eggs, colostrum, or bovine serum. They are unique in that they look very similar to Zonulin.  Look at it this way:

So as Zonulin comes back into the normal range we can then know when we can reduce or discontinue the treatment program with the gut barrier now intact. And this will now allow the chance to reintroduce many restricted foods back into the diet

Keep in mind that Zonulin is cited in the medical literature as related to the following:

In essence the Zonulin will tell us how leaky the gut really is and that is such a huge piece of information when I need to know how to guide a patient toward wellness

Diamine Oxidase – DAO

Zonulin gives us incredible insight into whether a gut lining is damaged or leaky, but another parameter, Diamine Oxidase greatly adds to this picture.  DAO is the enzyme responsible for gobbling up and breaking down excess histamine found in the foods we eat.  Histamine is found in many common foods like strawberries, tomatoes and “leftover” foods from a day or two before.  In fact, through bacterial action, the longer we wait to consume these “leftover” foods, the more histamine is present.  So perhaps it is histamine that is the reason for the same cantaloup producing symptoms today that was not seen when consumed fresh yesterday.

In situations like this, when we find a low level of diamine oxidase and correct it, it can be an awesome turning point for many individuals suffering with food reactions and other metabolic disturbances in the body.

DAO is made in the microvilli, the fingertips of the gut lining.  As these microvilli get worn down with gut inflammation, they produce less and less DAO, resulting in higher and higher levels of histamine, leading to elevated levels of inflammation.  And this lining can be broken down by any number of factors that damage the gut.

I have mentioned Zonulin already as a primary way we get a leaky gut, when it tells the tight junctions to open, allowing things to flood into the body.   But there is another path to a leaky gut and that being erosion, as happens with the chronic, continual use of non-steroidal anti-inflammatory drugs like aspirin.  These can wear down the gut lining to the point of ulceration.

There are histamine receptors all over the body, eyes, nose gut, skin and even in the brain and any of these tissues will react with inflammation if the histamine is excessive in those tissues.  And in the brain, elevated histamine can contribute to anxiety and depression and worse, ADHD and schizophrenia. 

In the heart we have histamine receptors as well and when the DAO is low, it may result in increased levels of histamine, that contribute to something as uncomfortable as arrythmia.  Treatment for that has been shown to protect against a heart going out of rhythm in some cases.  I suspect the several million individuals with this condition might like to see if their condition would improve if they knew they had an underlying DAO problem.

Likewise, histamine may affect the joints, impairing their flexibility and increasing pain.  Correcting DAO levels when low, may in fact result in less pain and freedom of movement.  Patients may find that even a bountiful intake of beneficial anti-inflammatory supplements, attempting to target that inflammation, will not help when the DAO level is too low.           

In addition to progesterone that drops off before a women’s period, DAO decreases as well.  So, women with PMS symptoms, and headaches that are not responding to progesterone or other traditional support measures, their DAO level needs to be checked.  And when low, correction will often resolve the migraines and other PMS symptoms.  Inflammation may start in the gut, but it does not stay there – any tissue in the body can be a target.

To produce DAO, we need to be getting adequate amounts of Fish oils, Vitamin C, vitamin B6 and copper.  These are the nutrients needed to produce DAO.  When these are not adequate, and just as often because of a genetic variant in the genes designed to produce DAO, we will need to use the specific enzyme as well.  And we know if it is necessary by the Advanced Intestinal Barrier Assessment providing us that information.

So, while we measure DAO in the Advanced Intestinal Barrier Assessment, histamine is also measured and what we want to determine is the relationship between the DAO level and the histamine level. 

You see, one may have lower levels of DAO but if the histamine is also at the bottom of normal, it might then be concluded that their DAO is adequate for them.

Histamine Intolerance

Typical symptoms of histamine intolerance are:

Histamine intolerance might be more obvious with specific food triggers leading to asthma and arrhythmia, hypotension, urticaria (hives), and dysmenorrhea. When DAO or histamine is imbalanced, the main focus of treatment is to increase DAO, reduce histamine, and heal the gut.

Lipopolysaccharides (LPS)

There is a third way for the gut to become leaky – elevated levels of, Lipopolysaccharides or LPS, which are bacterial endotoxins and found in the outer membrane of gram-negative bacteria.  And these are usually pathogenic bacteria.

First, Zonulin tells tight junctions to open up – like a garage door opener – that opens up a way for our gut lining to become leaky

Secondly, one can pound on the garage door and beat it till it breaks apart – that is due to a low DAO and the resulting aftereffects of excessive histamine that cause a leaky gut

Then this third way is to pick and pry at the door to get in – that is lipopolysaccharides, LPS. They can erode and wear down the tight junctions or even break through the cell itself to exit the gut and enter the blood stream

Elevated levels of either LPS IgA, LPS-IgG or LPS-IgE, will increase inflammation, damage the gut lining, and cause leaky gut. These are the three types of antibodies that we make toward lipopolysaccharides.

And when these antibodies are elevated, it tells us that our immune system is strongly reacting to these bacteria in your gut, as they see them as threats to the health of our body, and particularly prone to damaging the gut.

But there is also concern when the levels are very low.  You see, we always have some bacteria in the gut that challenge the lining and the gut should have adequate immune readiness and sensitivity for a healthy response – just not an excessive response

Therefore, Low levels of these antibodies likely suggest that the body has been in a long-term fight and the immune system’s ability to mount any further response is severely compromised.  These are often patients with Inflammatory bowel disease, chronic gut pain or chronic diarrhea.

So, when the LPS is elevated, the immune system is still in the fight and we support it with natural antibiotics like Berberine which helps to kills off the LPS, strengthens the tight junctions, but does not affect the microbiome.

But when these antibodies are at bottom normal or even low, there is a need to go after the infection but also there is a need to support the immune system or to evaluate where the immune imbalance is at using the Lymphocyte Map test.  Using immunoglobulins and other supportive measures is critical when the immune response is faltering and needs nutritional support.

When we look at Zonulin, DAO, Histamine, and LPS we have a comprehensive evaluation of leaky gut, damaging factors to the gut lining, indications of pathogenic microbes and an underlying factor in intestinal issues and other metabolic disturbances from the brain to the joints.  All these factors can be measured, tracked and treated by the Advanced Intestinal Barrier Assessment.  These factors are often major players in promoting inflammation that are at the root of so many symptoms and complaints that patients have today

Ask for this test. I believe you will find it extremely helpful.